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KMID : 0616120120430040279
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 4 p.279 ~ p.285
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Simultaneous Analysis and Anti-allergic Effect of Mume Fructus
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Seo Chang-Seob
Ha Hye-Kyung
Lee Ho-Young
Lee Jun-Kyoung
Jung Da-Young
Lee Jin-Ah
Shin Hyun-Kyu
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Abstract
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The Mume Fructus (MF) has been used for relieves cough, arrests arrest chronic diarrhea, treat fluid depletion, and treat ascariasis in Korea. In this study, a high-performance liquid chromatography (HPLC) method was established for imultaneous determination of six main components of MF. Additionally, we were investigated the anti-inflammatory and anti-allergic effects of MF extract on lipopolysaccharide (LPS)-treated RAW264.7 cells and tumor necrosis factor (TNF)-α/interferon (IFN)-γ-treated HaCaT cells. The analytical column for separation was used a Gemini C18 column maintained at 40 o C. The mobile phase consisted of 1.0% (v/v) acetic acid in water (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 280 nm and 320 nm. We evaluated the inhibitory effect of MF extract on the production of inflammatory markers, nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated RAW264.7 cells and thymus- and activation-regulated chemokine (TARC/CCL17) in TNF-α/IFN-γ-treated HaCaT cells, respectively. We confirmed the genes expression related with TARC, macrophage-derived chemokine (MDC/ CCL22) and regulated on activation, normal T cell expressed and secreted (RANTES/CCL5) in HaCaT keratinocyte cells by MF extract. The contents of the five compounds in MF were 0.22-1.01 mg/g. Also, the MF extract show inhibition of about 78% and 75% on NO and PGE2 production at the concentration 1000 mg/mL in RAW264.7 cells. MF extract suppressed the hTARC level and genes expression such as TARC, MDC, and RANTES on TNF-α/IFN-γ-treated HaCaT cells.
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KEYWORD
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Mume Fructus , Simultaneous analysis , Anti-allergy , RAW264.7 cell
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